96 – well pre – filled kit Application Areas

1. Field of life science research

1. Molecular biology
   • In gene cloning and expression research, 96 – well pre – filled kits can be used for the construction and screening of a large number of gene cloning vectors. For example, when constructing a cDNA library, reagents in the kit can be used for reverse transcription reactions to convert mRNA into cDNA, followed by ligation, transformation, and other operations, and the target genes can be found through high – throughput screening.
   • For gene – editing technologies, such as the optimization and screening of the CRISPR – Cas9 system, the kits can be used to process multiple samples simultaneously to test the editing efficiency of different combinations of sgRNA (single – guide RNA) and Cas9 protein on target genes, accelerating the research and development process of gene – editing technologies.
2. Cell biology
   • In terms of cell proliferation and cytotoxicity testing, these kits can be used to simultaneously detect the effects of multiple drugs or different concentrations of drugs on cell growth. For example, in the screening of anti – cancer drugs, cancer cells are inoculated into 96 – well plates, and different pre – filled drugs or drug combinations are added. By detecting cell viability indicators (such as MTT assay, CCK – 8 assay, etc.), the anti – cancer effects of drugs are evaluated, and potential anti – cancer drugs are quickly screened.
   • In the study of cell signal transduction, the expression of multiple proteins or genes related to signal pathways can be analyzed simultaneously. For example, when studying the effects of different growth factors on the activation of multiple intracellular signal pathways (such as MAPK, PI3K – Akt, etc.), protein quantification detection (such as reagents related to Western blot) or gene expression analysis (such as reagents related to qRT – PCR) using the kits can efficiently obtain experimental data.

2. Field of medical diagnosis

1. Diagnosis of infectious diseases
   • For viral infectious diseases, such as influenza and COVID – 19 infections, 96 – well pre – filled kits can be used for large – scale serological testing. For example, by using pre – filled ELISA (enzyme – linked immunosorbent assay) reagents, virus – specific antibodies in patients’ sera are detected to achieve rapid diagnosis and large – scale population screening.
   • In terms of bacterial infectious diseases, such as tuberculosis and pneumonia, they can be used for bacterial culture and drug – susceptibility tests. Using pre – filled culture media and drug – susceptibility testing reagents, multiple clinically isolated strains are cultured and tested for drug sensitivity simultaneously, helping doctors select appropriate antibacterial drugs for treatment.
2. Diagnosis of hereditary diseases
   • In gene testing for single – gene hereditary diseases, the kits can be used for high – throughput screening of gene mutations. For example, for common single – gene hereditary diseases such as cystic fibrosis and thalassemia, gene – testing of a large number of patient samples is carried out by using pre – filled gene – amplification and – sequencing – related reagents to quickly identify pathogenic gene mutation sites and assist in clinical diagnosis.
   • In prenatal diagnosis of chromosomal – abnormality diseases, such as Down syndrome, fetal – free DNA in the peripheral blood of pregnant women is analyzed by using pre – filled fluorescence – quantitative PCR or gene – chip – testing reagents to achieve non – invasive prenatal diagnosis and improve diagnostic efficiency and accuracy.

3. Field of drug research and development

1. Discovery and verification of drug targets
   • In the process of finding new drug targets, 96 – well pre – filled kits can be used to screen and verify a large number of potential targets. For example, by constructing different cell models or protein – protein interaction systems in 96 – well plates and using pre – filled detection reagents (such as reagents related to fluorescence resonance energy transfer (FRET), enzyme – activity – testing reagents, etc.), the effects of various compounds on potential targets are evaluated to quickly discover targets with potential drug – development value.
2. Screening and optimization of drug activity
   • In the drug – activity – screening stage, thousands of compounds can be preliminarily screened simultaneously. For example, compounds from a compound library are added to different wells of a 96 – well plate respectively, combined with pre – filled target proteins or cell models and corresponding detection reagents, and compounds that can bind to targets or have expected pharmacological effects on cells are quickly screened.
   • In the process of drug optimization, the kits can be used to conduct further structure – activity relationship (SAR) studies on the screened potential compounds. By setting different compound concentrations or structural modifications in different wells and combining with pre – filled activity – testing reagents, the optimal drug structure and dosage are determined to improve the efficiency of drug research and development.

4. Field of environmental science

1. Detection of environmental pollutants
   • In detecting pollutants in water bodies, 96 – well pre – filled kits can be used to detect heavy metal ions, organic pollutants, etc. in water. For example, by using pre – filled chemical – color – developing reagents or immuno – testing reagents, rapid qualitative and quantitative analyses of heavy metals (such as mercury, lead, cadmium, etc.) or organic pollutants (such as polycyclic aromatic hydrocarbons, pesticide residues, etc.) in water samples are carried out to evaluate the degree of water pollution.
   • For the detection of soil pollutants, high – throughput detection of harmful substances in soil can be carried out. For example, detecting petroleum hydrocarbon pollutants, persistent organic pollutants, etc. in soil to provide data support for soil – pollution treatment and remediation.
2. Research on microbial ecology
   • In studying the structure and function of environmental – microbial communities, the kits can be used for high – throughput culturing and identification of microorganisms in environmental samples such as soil and water. For example, by using pre – filled microbial – culture media and – identification reagents, microorganisms in environmental samples are cultured and identified based on 16S rRNA genes or other functional genes, and the diversity and composition of microbial communities are analyzed to understand the roles of environmental microorganisms in ecosystems.