HCY™ HotStart SYBR Green qPCR Master Mix Premix PCR Enzyme

HCY™ HS-Taq DNA Polymerase is based on HCY™ Taq DNA Polymerase modified by a special chemical modification method.HCY™ HS-Taq DNA Polymerase possesses 5′-3′ DNA polymerase activity and 5′ -3′ exonuclease activity and no 3′-5′ exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.
HCY™ HS-Taq DNA Polymerase activity is activated only after 10 minutes of pre-denaturation at 98°C, which inhibits non-specific amplification caused by non-specific annealing of the primers or primer dimerization at low temperatures. The PCR reaction with this product can be operated at room temperature, and can effectively avoid the non-specific PCR background caused by the activity of common Taq enzyme or other heat-resistant DNA polymerase during room temperature operation.

HCY™ HotStart SYBR Green qPCR Master Mix (2×) is suitable for the detection of Real Time PCR qualitative and quantitative reactions using SYBR Green I chimeric fluorescence method.
It contains all common components such as HS-Taq DNA Polymerase, dNTPs, Mg2+, SYBR Green I fluorescent dye, etc. It is only necessary to add template, primers and water for PCR amplification. This product adopts the optimized formulation of qPCR special Buffer, which can get a good standard curve in a wide quantification region and accurately perform quantification. This product is compatible with most manufacturers’ fluorescence quantitative PCR instruments, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche.
This product contains individually packaged ROX for use in PCR instruments that require ROX as a correction dye, which is designed to minimize well-to-well variation by correcting for fluorescence fluctuations unrelated to PCR. Such differences can be caused by a variety of factors, such as pipetting errors and sample evaporation. Different quantitative PCR instruments have different requirements for ROX, so please select the concentration of ROX to be added according to the actual instrument used.

HCY™ HS-Taq DNA Polymerase specificity
1 is HCY™ HS-Taq DNA Polymerase, 2 is Taq DNA Polymerase from other companies, the amplified fragments (500bp-5000bp), it can be seen that there is no heterozygous band in HCY™ HS-Taq DNA Polymerase, and the hot-start can effectively prevent non-specific amplification.
M stands for DL10,000 DNA Marker.
Quality Control
The purity of SDS-PAGE electrophoresis is greater than 98%;
Functional test: sensitivity, specificity and reproducibility of PCR;
No exogenous nuclease activity, no exogenous endonuclease or exonuclease contamination;
No expression host nucleic acid residue.
Precautions: This product is for scientific research use only, and cannot be used for medical or diagnostic procedures in humans or animals, or as food, cosmetics or household products, etc. Without written permission authorization or approval, this product may not be manufactured, licensed for sale, sold, or imported or exported, or use all related patents and related trademarks of the product.