Advantages of cell preservation solution

Advantages of cell preservation solution

Currently, most of the protective agents for cryopreservation of cells are glycerol or dimethyl sulfoxide. In fact, these two substances can increase the permeability of cell membranes to water. In addition, slow freezing can make the water in the cell seep out of the cell and reduce the ice crystals in the cell. The formation of ice crystals reduces cell damage caused by the formation of ice crystals.

Cells should be resuscitate by a fast melting method, which can ensure that the extracellular crystals melt in a short time,

and avoid damage to the cells due to the infiltration of water into the cells to form intracellular recrystallization; non-program cooling, -80℃ Cryopreservation in a low-temperature refrigerator for up to 3 years can ensure the safety of cryopreserved cells; cryopreservable plates (cell culture plates) can be use for cryopreservation of hybridoma cells. The cell preservation solution has a diluting effect and can be separated.

A large number of effective cells embed in the mucus allows more cells of test value to be preserve, providing a sufficient number of cells to ensure the accuracy of the test results;

at the same time, the process samples can be completely remove after low-speed centrifugal filtration The mucus in the sample effectively prevents the mucus from interfering with the test results of the sample.

The cell preservation solution can be make into a uniform monolayer cell sheet after low-speed centrifugation,

which meets the inspection requirements.

summary

Cell preservation solution is a general-purpose cell cryopreservation solution that can be use to freeze cell lines of humans and various animals; cell cryopreservation is an important technical means for cell culture, introduction, preservation and ensuring the smooth progress of experiments. In cell line establishment and line establishment, it is very important to freeze the original cells in time. In the preparation process of hybridoma monoclonal antibodies,

the cryopreservation of hybridoma cells and subcloned cells obtained from each cloning is often an indispensable experimental operation.

Because when a stable cell line or a cell line that secretes antibodies is not established,

the cell culture process may cause the experiment to fail due to cell contamination,

loss of antibody secretion ability or genetic variation, etc ,

if there is no original cell Frozen storage will be abandoned because of the above-mentioned accidents.

Advantages of cell preservation solution