Cell culture medium

Cell culture medium is a liquid environment used to maintain and support cell growth, proliferation and survival.
It usually contains a variety of nutrients, such as amino acids, vitamins, carbohydrates, inorganic salts, etc., to provide cells with the necessary energy and material base. At the same time, cell culture medium also has the function of regulating pH value, osmotic pressure, etc., to ensure that the cells grow under appropriate conditions.

Cell culture media are mainly categorized in the following categories:
Natural media: made from extracts of animal, plant or microbial bodies.
Synthetic media: formulated from substances of well-defined chemical composition.
Serum-free media: media that do not contain animal serum.
Limited chemical composition media: the composition is more defined and precise.

Overview of cell culture media:
Cell culture medium is the material basis for providing a suitable environment for cell growth, proliferation and maintenance.
Main ingredients:
Nutrients: including amino acids, vitamins, carbohydrates (e.g. glucose), inorganic salts, etc., which provide cells with energy and substances necessary for building cells.
Growth factors: promote cell growth, differentiation and functional expression.
Buffering system: maintains pH stability of the culture medium.
Other components: e.g. chelating agents, antioxidants, etc.
Role:
Provide nutrients: meet the various nutrients required for cell growth.
Maintaining environment: Maintaining appropriate osmotic pressure, pH and gas conditions.
Promote growth: support cell proliferation, differentiation and functional expression.
Classification: natural medium, synthetic medium, serum-free medium, etc., as mentioned earlier.
Applicability to different cell types: Different cells have different requirements for culture media, and it is necessary to choose suitable culture media according to the characteristics of cells.
Application: widely used in cell biology research, cell culture production and other fields.