Cell culture medium has a wide range of specific applications, which are mainly as follows:

Basic Research

• Cell Biology Research: It provides an in vitro growth environment for cells, helping researchers study the basic physiological processes of cells, such as cell growth, proliferation, differentiation, metabolism, and signal transduction, to understand the behavioral characteristics of cells under normal and pathological conditions.
• Molecular Biology Research: It is used to culture cells for the extraction of biological macromolecules like DNA, RNA, and proteins, enabling gene expression analysis, gene regulation research, and proteomics research, so as to reveal the interactions and functions between genes and proteins.
• Research on Disease Mechanisms: By culturing cancer cells and diseased cells, it helps to deeply study the occurrence and development mechanisms of diseases, such as the metastasis mechanism of cancer and the pathological processes of neurodegenerative diseases, providing a theoretical basis for disease diagnosis and treatment.

Biopharmaceutical Industry

• Antibody Production: It is crucial for the production of monoclonal and polyclonal antibodies. It provides a suitable growth environment for hybridoma cells or immune cells, enabling them to synthesize and secrete antibodies in large quantities for disease diagnosis, treatment, and research.
• Vaccine Production: Many viral vaccines are produced by culturing viruses in cell culture medium and then subjecting them to inactivation, attenuation, or other treatments. For example, influenza vaccines and hepatitis B vaccines are made in this way. The cell culture medium provides the necessary environment for virus replication and proliferation.
• Production of Gene Therapy Vectors: It is used to culture and produce viral or non-viral vectors required for gene therapy, such as adenovirus vectors and lentivirus vectors, to introduce therapeutic genes into patients’ bodies and achieve the purpose of gene therapy.

Cell Therapy

• Stem Cell Culture: Stem cell culture medium can maintain the self-renewal and multi-directional differentiation abilities of stem cells. It is used for the culture and amplification of embryonic stem cells, induced pluripotent stem cells, etc., providing a sufficient number of stem cells for cell therapy.
• Immune Cell Culture: It is used to culture immune cells such as T cells, NK cells, and CAR-T cells for adoptive immunotherapy, enhancing patients’ immune functions and treating tumors, infectious diseases, and so on.

Tissue Engineering

• Cell Culture and Tissue Construction: It provides an environment for the growth and differentiation of seed cells in tissue engineering, enabling them to proliferate on scaffold materials and form tissues with specific functions, such as bone tissues, cartilage tissues, and skin tissues, which are used for tissue repair and regeneration.
• Organ Model Construction: By culturing multiple cell types in three-dimensional culture medium, organoid models are constructed to simulate the structures and functions of human organs, which are used for drug screening, disease research, and toxicology research.

Clinical Diagnosis

• Pathogen Detection: It is used to culture pathogens such as bacteria, fungi, and viruses. By observing the growth characteristics and morphological structures of the pathogens, identification and diagnosis are carried out to determine the source of infection and the cause of illness.
• Tumor Cell Detection: Tumor cells from cancer patients are cultured in vitro for drug sensitivity tests, providing references for clinicians to select appropriate chemotherapy drugs and improving the treatment effect.

Agriculture and Animal Husbandry and Veterinary Medicine

• Plant Cell Culture: In plant tissue culture, the medium is used to culture plant cells, tissues, or organs to achieve rapid plant propagation, virus-free culture, genetic transformation, etc., and to cultivate excellent varieties.
• Animal Cell Culture: It is used to culture animal cells, such as in the production of animal vaccines and animal cell engineering research. It can also be used to detect animal viruses and study the pathogenesis of animal diseases.