HCY™ HotStart Taqman qPCR Master Mix(2×, No Rox)

HCY™ HotStart Taqman qPCR Master Mix (2×, No Rox) Premix DNA PCR Enzyme
HCY™ HS-Taq DNA Polymerase is based on HCY™ Taq DNA Polymerase modified by a special chemical modification method.HCY™ HS-Taq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. ‘-3′ exonuclease activity and no 3′-5′ exonuclease activity. The product carries an A base at the 3’ end and can be directly cloned with TA vector.HCY™ HS-Taq DNA Polymerase activity is activated only after 10 minutes of pre-denaturation at 98°C, which can inhibit non-specific amplification caused by non-specific annealing of the primers or primer dimerization under low temperature conditions. HCY™ HotStart Taqman qPCR Master Mix (2×) is suitable for Real Time PCR qualitative and quantitative assays using the probe method. reaction using the probe method.
This product uses optimized formula of qPCR special buffer, which can get a good standard curve in a wide quantification region and accurate quantification. This product is compatible with most manufacturers’ fluorescence quantitative PCR instruments, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche.
This product contains individually packaged ROX for use in PCR instruments that require ROX as a correction dye, which is designed to minimize well-to-well variation by correcting for fluorescence fluctuations unrelated to PCR. Such differences can be caused by a variety of factors, such as pipetting errors and sample evaporation. Different PCR instruments require different concentrations of ROX, so please select the concentration of ROX to be added according to the actual instrument used.

Storage conditions
Avoid strong light when storing or preparing the reaction system. For long term storage, please store at -20℃ and keep away from light. If frequent use is required, it can be stored at 4℃ after dispensing, avoiding repeated freezing and thawing. After thawing, the product should be mixed thoroughly to avoid the generation of large amounts of air bubbles.