Nucleic acid aerosol contamination remover

Aerosol is suspended in the gas medium particle size is generally 1nm ~ 1mm solid, liquid micro-particles formed by the colloidal state of the bulk system, which is dispersed and suspended in the gas medium of nucleic acid polymers, widely available in the laboratory desktop, instruments, consumables, as well as in the air.
Nucleic acid aerosols go hand in hand with nucleic acid amplification processes (PCR, etc.). DNA aerosols can be generated by friction between air and liquid surfaces, centrifugation in centrifuges, violent shaking of reaction tubes, PCR uncapping, repeated pipetting, and leakage of contaminants, among other pathways. Molecular laboratories as research and clinical testing sites, PCR is used more frequently, and the samples and amplification targets are often the same in multiple batches, nucleic acid aerosol contamination accumulates in the experimental area, the risk of contamination increases, and the frequency of false positives increases. the high amplification efficiency of PCR and other technologies produces nucleic acid aerosol contamination, which can lead to false-positive test results. False positives mean that the experiment is not trustworthy and directly results in financial losses for the laboratory. More seriously, if aerosol contamination is formed, it can cause contamination of the entire PCR laboratory, or even the closure of the laboratory.
Due to the high heat resistance of DNA, high adsorption capacity and high tolerance to organic solvents, high temperature, high pressure, UV irradiation and ethanol wipe can only degrade DNA to 50~500 bp length, which is not able to eradicate DNA contamination. Brilliance Sun’s newly developed HCY-Cleanase can degrade any form of DNA and RNA (double-stranded, single-stranded, linear, cyclic, natural or denatured) into 5′-monophosphate oligonucleotides of 3~8 bases in 15min at room temperature, whether it is pipettes, PCR instruments, or consumables, nucleic acid adsorbed contaminants on the laboratory desktop are enzymatically degraded. After the nucleic acids are enzymatically deactivated, HCY-Cleanase can be irreversibly inactivated by drying at 60℃ for 10min or by wiping with an enzyme activity eliminator, thus avoiding subsequent effects on the experiment. This product does not contain any organic toxic solvents, non-toxic, non-corrosive, and does not cause any damage to the equipment.

Storage conditions:

Unopened -20℃ can be stored for 3 years
The enzyme and buffer should be used as it is prepared, if it is not used up at one time, it can be stored at 4℃ for 1 month, avoiding repeated freezing and thawing.
Usage:

I. High temperature drying equipment

The equipment that can be dried at 60℃ includes pipette, gun-tip box, 96-well plate, ice box, forceps, scalpel, palm centrifuge and so on.
Add ddH2O or 70% ethanol to the spray bottle 1, spray the instruments 2~3 times, and wipe clean with a clean towel or dust-free paper to remove the surface stains.
Pour Enzyme Buffer A into Spray Bottle 2, add 100 μl of HCY-Cleanase into Spray Bottle 2 (ready to use), and mix well by shaking vigorously. Spray onto the corresponding instruments, subject to the uniform coverage of water droplets. Leave the sprayed instruments at room temperature (25~37℃) for 15~30min (the optimal action temperature of HCY-Cleanase is 35℃, the enzyme activity decreases sharply above 45℃).
After the DNA digestion was completed, the instruments were sprayed with ddH2O or 70% ethanol spray bottle 1 for 2~3 times and wiped clean with a clean towel or dust-free paper, and placed in the oven at 60℃ for 10min to inactivate HCY-Cleanase. The inactivated instruments can be used normally.

Second, not high temperature drying equipment

This type of equipment includes: PCR instruments, laboratory bench tops, centrifuges, ultra-clean tables, etc.
Add ddH2O or 70% ethanol to a spray bottle1, spray the equipment several times, and wipe it off with a clean towel or dust-free paper. Turn the laboratory ventilation on to displace the air in the room.
Pour Enzyme Buffer A into Spray Bottle 2, add 100 μl of HCY-Cleanase into Spray Bottle 2 (ready to use) and mix well by shaking vigorously. Spray onto the corresponding instruments or countertops, subject to uniform coverage of water droplets. Leave the sprayed instruments at room temperature (25~37℃) for 15~30min (the optimal action temperature of HCY-Cleanase is 35℃, the activity of the enzyme decreases sharply above 45℃).
After DNA digestion was completed, Enzyme Buffer B was poured into the spray bottle 3 and sprayed 2~3 times on the instrument or table surface, waiting for 3-5 minutes to completely inactivate HCY-Cleanase, and wiped off with a clean towel or dust-free paper. Then use the spray bottle 1 containing ddH2O or 70% ethanol to spray the instrument or tabletop, and wipe it off with a clean towel or dust-free paper. The instrument or countertop is ready for normal use.
III. Indoor space

In case of serious pollution, it is necessary to spray the indoor air to remove the pollutants in the air, the dosage is 100ml of this product per 40m3 space, close the door, turn off the ventilation and wait for 30min. be careful to put the equipment in the state of power off to avoid causing fire.
Spray an equal amount of Enzyme Buffer B into the room, close the door, shut off the ventilation and wait for 10min.
After spraying, wipe the surface with ddH2O or 70% ethanol and open the ventilation equipment or doors and windows.

Precautions

HCY-Cleanase is a powerful all-purpose nucleic acid degrading enzyme, safe and non-toxic. However, it is still recommended to wear appropriate gloves, masks and other protective devices during operation. HCY-Cleanase is highly sensitive to temperature, the surface temperature of the instrument to be decontaminated must be lower than 45 ℃, and the optimal reaction temperature is 35 ℃.
HCY-Cleanase is not sensitive to light and ethanol, and does not require a high environment for its use. However, enzyme deactivators and high temperatures can irreversibly deactivate HCY-Cleanase, so it has no effect on downstream applications after the instruments have been sterilised.
HCY-Cleanase acts on any form of DNA and RNA (double-stranded, single-stranded, linear, circular, natural or denatured) and degrades it into 5′-monophosphate oligonucleotides of 3 to 8 bases in length.
This product does not contain any organic toxic solvents, is non-toxic, non-corrosive and does not cause any damage to equipment. However, it is still recommended to wipe the surface with ddH2O or 70% ethanol after using this product.
This product is for research use only, please do not use it for human and animal treatment, clinical diagnosis or as an additive in food, cosmetics, household products and other purposes.