The Outstanding Contribution of Cell Culture Medium in the Biopharmaceutical Field”

In the cutting-edge and crucial field of biopharmaceuticals, cell culture medium plays an irreplaceable and important role.

Its specific applications are extremely extensive. In terms of antibody production, it provides an ideal growth microenvironment for hybridoma cells or recombinant engineered cells. These cells can efficiently carry out metabolism, proliferation, as well as antibody synthesis and secretion in the culture medium. For example, the large-scale industrial production of monoclonal antibodies relies heavily on high-quality cell culture medium, which ensures the yield and quality of antibodies, enabling numerous therapeutic antibody drugs to be widely used in the treatment of cancers, autoimmune diseases, and so on. In the field of vaccine manufacturing, whether it’s traditional inactivated vaccines, live attenuated vaccines, or emerging recombinant subunit vaccines, cell culture medium is the foundation for the cultivation and amplification of pathogens such as viruses and bacteria. For instance, in the production of influenza vaccines, influenza viruses are cultured in specific cell culture media, followed by subsequent processing techniques, to produce vaccine products that can be used for influenza prevention among the global population.

Cell culture medium has many remarkable advantages. Firstly, it has a precise ratio of nutritional components. Based on the requirements of different cell types, it precisely contains various nutrients such as amino acids, vitamins, and sugars, ensuring that cells can obtain sufficient and appropriate nutrient supply to promote rapid cell growth and high-activity expression. Secondly, it has excellent buffering capacity and can stably maintain the pH value within a relatively narrow range during the cell culture process. This is crucial for maintaining the normal physiological functions of cells and avoids cell growth inhibition or death caused by pH fluctuations. Moreover, some advanced cell culture media are customizable and can be optimized and adjusted according to specific cell lines or production process requirements, further improving the efficiency of cell culture and the quality of the products.

However, there are also some precautions when using cell culture medium. The storage conditions of the medium should be strictly controlled. Generally, it needs to be stored in a low-temperature and dark environment to prevent the degradation and deterioration of nutritional components. Before use, the appearance of the medium should be carefully inspected. If there are any abnormal phenomena such as turbidity or precipitation, it should not be used. In addition, during the cell inoculation process, attention should be paid to the control of inoculation density. Either too high or too low inoculation density may affect the growth state of cells and the synthesis of products. Meanwhile, the metabolic situation during the cell culture process should be closely monitored. Fresh medium should be supplemented or the culture conditions should be adjusted in a timely manner according to the growth stage of cells to ensure the smooth progress of cell culture and the efficient production of target products.