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Virus preservation liquid contains the following common buffer systems:
Virus preservation liquid contains the following common buffer systems:
Hanks buffer
- Component: Contains various inorganic salts and glucose and other nutrients, such as sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, etc.
- Characteristic: It can provide a relatively stable physiological environment for the virus, maintain the activity and stability of the virus. Its components are similar to the extracellular fluid in the body, which is conducive to maintaining the physiological state of the virus and reducing the impact of the external environment on the virus.
- Application: Commonly used in non-inactivated virus preservation liquid, which can be used for the collection and transportation of virus specimens such as clinical influenza, avian influenza, hand-foot-mouth disease, and measles, as well as specimens of mycoplasma, ureaplasma, and chlamydia.
Tris buffer
- Component: Tris (hydroxymethyl) aminomethane and its hydrochloride, with a pH value between 7.0 and 9.0.
- Characteristic: It has good buffering ability, can effectively maintain the stability of the pH value of the solution, and has a certain protective effect on the stability of viral nucleic acid. And it can maintain stable buffering performance within a wide temperature range, and can resist the influence of temperature changes on the pH value to a certain extent.
- Application: It is more commonly used in some virus preservation liquids that need to maintain the integrity of viral nucleic acid, such as virus preservation liquids for nucleic acid detection, which can help ensure the accuracy of subsequent nucleic acid extraction and detection.
PBS buffer
- Component: Mainly composed of potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, and potassium chloride, etc.
- Characteristic: It is similar to the osmotic pressure of the human physiological environment, has a small osmotic pressure impact on the virus, helps maintain the structural and functional integrity of the virus. It has good ionic strength and buffering performance, can stabilize the pH value of the solution, and reduce the damage to the virus caused by acid-base changes.
- Application: Widely used in the preservation and processing of various biological samples, including virus samples. It is suitable for the preservation of various virus types, especially for viruses that are sensitive to osmotic pressure.
BES-POPSO buffer
- Component: Composed of BES [N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid] and POPSO (piperazine-1,4-dihydroxypropane sulfonic acid).
- Characteristic: It can maintain a relatively stable pH value within a certain temperature range, provide a suitable living environment for the virus, and can effectively resist the interference of external factors on the pH value, ensuring the stability of the virus preservation liquid.
- Application: It can be used in some situations that require high preservation conditions and need long-term stable preservation of the virus, such as in specific scientific research experiments or long-term sample storage.
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