Monkeypox virus

The following is a more detailed description:

Characteristics and Advantages

• Rapidness: Some rapid test kits for monkeypox virus generally can give results within 15-20 minutes, which can quickly determine whether there is an infection, facilitating timely prevention and control measures. And like the nucleic acid detection kit for monkeypox virus (fluorescent PCR method) combined with various types of fluorescent PCR instruments, it can carry out efficient amplification and improve the detection efficiency, and can obtain accurate results in a relatively short time, which is suitable for large-scale screening and rapid diagnosis.
• High specificity: The nucleic acid detection kit for monkeypox virus takes the highly conserved region in the F3L gene coding region of the monkeypox virus (MPXV) as the target area, and designs specific primers and fluorescent probes to carry out PCR amplification, which can accurately identify the monkeypox virus, reduce the risk of missed detection, and the detection result is accurate and reliable, effectively avoiding cross-reaction with other similar viruses.
• High sensitivity: It can detect extremely low concentrations of viral nucleic acids, and even if there is less viral content in the sample, it can accurately detect it, which is conducive to the early detection of infected people and is of great significance for controlling the spread of the epidemic.
• Internal reference monitoring: Some kits include an endogenous internal reference (RNase P gene) detection system, which can monitor the collection of specimens, nucleic acid extraction process, and PCR amplification process, and detect possible problems in time to reduce the occurrence of false-negative results and improve the accuracy of detection.
• Pollution prevention and control: The kit adds UDG enzyme and dUTP anti-pollution components to effectively reduce the occurrence of false positive results caused by PCR product pollution and ensure the authenticity of the detection result.
• Compatibility: Such as the nucleic acid detection kit for monkeypox virus is compatible with multiple PCR platforms, facilitating its application in different laboratory environments, and is not limited by specific instrument equipment, and can be widely used in medical institutions at all levels and professional testing laboratories.

Precautions

• Sample collection:
  • Collection time: It should be collected at an appropriate time after infection to improve the positive rate of detection. Generally speaking, when symptoms such as fever and rash appear in the early stage, it is more appropriate to collect samples from skin lesions or throat swabs.
  • Collection method: Different sample types have different collection methods, which need to be strictly carried out in accordance with the instructions of the kit or relevant standard operating procedures. For example, samples of skin lesions can be sampled by gently wiping with a swab on the surface of the rash or exudate; throat swabs need to be wiped and collected in the throat area, etc.
  • Sample preservation and transportation: If the collected samples cannot be detected in time, they need to be preserved and transported according to requirements. Usually, they need to be stored under specific temperature conditions and use appropriate transportation media to ensure the stability and activity of the samples and prevent the degradation or inactivation of viral nucleic acids in the samples.
• Operation process:
  • Operate strictly according to the instructions: The operation steps of the detection reagent are relatively strict, and the addition of samples, incubation, centrifugation and other operations should be carefully read and strictly carried out in accordance with the instructions of the kit. Any mistake in any link may affect the accuracy of the detection result.
  • Prevent cross-contamination: During the operation, it is necessary to pay attention to using one-time pipette tips, test tubes and other consumables to avoid cross-contamination. When drawing different reagents, pipette tips should be replaced to prevent mutual contamination between reagents; at the same time, the experimental tabletop, instrument equipment, etc. should also be kept clean and disinfected regularly.
  • Control the reaction conditions: Reaction conditions such as temperature and time are crucial to the detection result. It is necessary to ensure the accuracy and stability of the incubation equipment temperature, and strictly carry out the reaction according to the specified time to ensure the specificity and sensitivity of the detection.
• Interpretation of results:
  • Pay attention to the timeliness of results: The detection results should be observed and recorded within the specified time. Exceeding the observation time may lead to inaccurate or difficult to judge results.
  • Combine with clinical symptoms for judgment: The detection result is only one of the reference bases for diagnosis, and the infection of monkeypox virus cannot be diagnosed or excluded only by the detection result. Even if the detection result is negative, but if the patient has typical clinical symptoms and epidemiological history, further examination and observation are still required; conversely, if the detection result is positive, it also needs to be comprehensively judged in combination with clinical symptoms, etc.

Composition Principle

• Nucleic acid detection reagent:
  • PCR amplification principle: The nucleic acid detection kit for monkeypox virus is mainly based on the polymerase chain reaction (PCR) technology. PCR is a molecular biological technology used to amplify specific DNA fragments. Its principle is to dissociate the double-stranded DNA into single-stranded at high temperature, then primers bind to the single-stranded DNA template at low temperature, and then new DNA chains are synthesized by the catalysis of DNA polymerase at an appropriate temperature, and so on. By designing specific primers, it can selectively amplify a specific gene fragment of the monkeypox virus, such as the F3L gene, in the PCR reaction.
  • Fluorescent probe detection: A fluorescent probe is added during the PCR amplification process. The fluorescent probe is an oligonucleotide probe with a fluorescent label, and its sequence is complementary to a certain area inside the target DNA fragment. When the PCR reaction is carried out, as the target DNA fragment is amplified, the fluorescent probe will bind to the amplified product, and emit a fluorescent signal under specific fluorescent excitation. By real-time monitoring the intensity change of the fluorescent signal, the progress and result of the PCR amplification can be understood in real time, thereby realizing the quantitative or qualitative detection of the nucleic acid of the monkeypox virus.
• Antigen detection reagent: The monkeypox virus detection test paper is based on the principle of antigen-antibody reaction. The specific antigen of the monkeypox virus is fixed on the test paper. When there is the monkeypox virus in the sample, the antigen on the surface of the virus will bind to the antibody on the test paper to form an antigen-antibody complex, so that a color change or other visible signal appears in the detection area on the test paper, thereby judging whether the sample contains the monkeypox virus.