PREPARATION OF VIRAL TRANSPORT MEDIUM

PREPARATION OF VIRAL TRANSPORT MEDIUM

ARATION OF VIRAL TRANSPORT MEDIUM,This SOP was adapted from the Indiana University (IU) Health Clinical Virology Laboratory (Indianapolis, IN)
protocol entitled Preparation of Viral Transport Medium and Cell Culture Media, which was developed by Mr. Fred
Joyce (dec.) and shared with the clinical microbiology and public health laboratory communities via private listserv on
March 13, 2020 by Dr. Ryan F. Relich, IU Health Clinical Virology Laboratory Director, and laboratory staff.

1.0 Purpose

To provide a standard operating procedure (SOP) for producing viral transport
medium (VTM) for specimens for viral culture or other means of viral detection.
This SOP provides an alternative to commercially available products. There are
many acceptable formulations of this medium that may be suitable for the unique
conditions of individual laboratories. The specific needs of the shipping and
receiving laboratories should be considered when choosing a VTM formulation.

2.0 Scope

This document applies to VTM produced in support of the Centers for Disease Control and Prevention (CDC) Coronavirus Disease 2019 (COVID-19) outbreak response.

3.0 Responsibility

It is the responsibility of personnel preparing VTM for CDC’s COVID-19 outbreak response to follow this SOP accurately. If there are variations from this SOP, the variations should be documented, and data generated to demonstrate equivalent performance of the VTM.

4.0 Definitions

4.1 DOM – Date of Manufacture

5.0 References

5.1 Leland, D.S. 1992. Concepts of clinical diagnostic virology, p. 3-43. In E.H. Lennette (ed.), Laboratory Diagnosis of Viral Infections, second edition. Marcel Dekker, Inc., New York.
5.2 Johnson, F.B. 1990. Transport of Viral Specimens. Clinical Microbiology Reviews 3(2):120-131.
5.3 Biosafety in Microbiological and Biomedical Laboratories (BMBL), current edition
5.4 CLSI Standard, M40-A2; Quality Control of Microbiological Transport Systems; Approved Standard-Second Edition
5.5 Smith, K.P. 2020. Large-Scale, In-House Production of Viral Transport Media to Support SARS-CoV2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic. Journal of Clinical
Microbiology, volume 58, issue 8 5.6 Joyce, Fred. Preparation of Viral Transport Medium and Cell Culture Media. Indiana University (IU) Health Clinical Virology Laboratory

PREPARATION OF VIRAL TRANSPORT MEDIUM.PDF

This document applies to VTM produced in support of the Centers for Disease
Control and Prevention (CDC) Coronavirus Disease 2019 (COVID-19) outbreak